PDBe 3eg9

X-ray diffraction
3Å resolution

Crystal structure of the mammalian COPII-coat protein Sec23/24 bound to the transport signal sequence of membrin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein transport protein Sec23A Chain: A
Molecule details ›
Chain: A
Length: 764 amino acids
Theoretical weight: 86.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15436 (Residues: 1-764; Coverage: 100%)
Gene name: SEC23A
Sequence domains:
Structure domains:
Protein transport protein Sec24D Chain: B
Molecule details ›
Chain: B
Length: 770 amino acids
Theoretical weight: 86.65 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O94855 (Residues: 266-1032; Coverage: 74%)
Gene names: KIAA0755, SEC24D
Sequence domains:
Structure domains:
Golgi SNAP receptor complex member 2 Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 764 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O14653 (Residues: 116-121; Coverage: 3%)
Gene names: GOSR2, GS27

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 103.13Å b: 140.75Å c: 152.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.254
Expression systems:
  • Spodoptera frugiperda
  • Not provided