› 3edf

X-ray diffraction
1.65Å resolution

Structural base for cyclodextrin hydrolysis

Released:
DOI: 10.2210/pdb3edf/pdb
PDB entry 3edf coloured by chain, front view.
PDB entry 3edf coloured by chain, side view.
PDB entry 3edf coloured by chain, top view.
Source organism: Flavobacterium sp. 92
Primary publication:
Structural base for enzymatic cyclodextrin hydrolysis.
Buedenbender S, Schulz GE
J Mol Biol 385 606-17 (2009)
PMID: 19014948

Function and Biology Details

Reaction catalysed: 
Cyclomaltodextrin + H(2)O = linear maltodextrin
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185144 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 601 amino acids
Theoretical weight: 68.02 KDa
Source organism: Flavobacterium sp. 92
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KKG0 (Residues: 19-619; Coverage: 100%)
Gene name: cdase
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand CA 4 x CA
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 106.542Å b: 111.107Å c: 106.602Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.167 0.201
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Cyclodextrins.
Kurkov et al. (2013)
5 more

2 mentions without citation

The carbohydrate-active enzymes database (CAZy) in 2013.
Lombard et al. (2014)
1 more