› 3ed8

X-ray diffraction
2.7Å resolution

Application of the superfolder YFP bimolecular fluorescence complementation for studying protein-protein interactions in vitro

Released:
DOI: 10.2210/pdb3ed8/pdb
PDB entry 3ed8 coloured by chain, front view.
PDB entry 3ed8 coloured by chain, side view.
PDB entry 3ed8 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Applicability of superfolder YFP bimolecular fluorescence complementation in vitro.
Ottmann C, Weyand M, Wolf A, Kuhlmann J, Ottmann C
Biol Chem 390 81-90 (2009)
PMID: 19007309

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 260 amino acids
Theoretical weight: 29.34 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-154, 155-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 5 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43212
Unit cell:
a: 123.04Å b: 123.04Å c: 247.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.218
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.
Romei et al. (2019)
3 more

2 mentions without citation

Method of Monitoring 26S Proteasome in Cells Revealed the Crucial Role of PSMA3 C-Terminus in 26S Integrity.
Steinberger et al. (2023)
1 more