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Structure of N141A HincII with Cognate DNA

X-ray diffraction
2.55Å resolution
PDB entry 3ebc coloured by chain, front view.
PDB entry 3ebc coloured by chain, side view.
PDB entry 3ebc coloured by chain, top view.
Source organism: Haemophilus influenzae
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Type II restriction enzyme HincII and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 23351.01 Å2
Buried surface area: 8735.94 Å2
Dissociation area: 2,447.38 Å2
Dissociation energy (ΔGdiss): 45.95 kcal/mol
Dissociation entropy (TΔSdiss): 13.55 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-116203

Macromolecules

Type II restriction enzyme HincII
Chains: A, B
Length: 317 amino acids
Theoretical weight: 36.59 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 1-258; Coverage: 100%)
Gene name: hincIIR
Pfam: Restriction endonuclease HincII
InterPro:
CATH: DNA mismatch repair MutH/Restriction endonuclease, type II
Type II restriction enzyme HincII in PDB entry 3ebc, assembly 1, front view.
Type II restriction enzyme HincII in PDB entry 3ebc, assembly 1, side view.
Type II restriction enzyme HincII in PDB entry 3ebc, assembly 1, top view.
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DNA chain E
Name: 5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DGP*DGP*DGP*DC)-3'
Representative chains: E
Source organism: Haemophilus influenzae [727]
Expression system: Not provided
Length: 14 nucleotides
Theoretical weight: 4.32 KDa
5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DGP*DGP*DGP*DC)-3' in PDB entry 3ebc, assembly 1, front view.
5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DGP*DGP*DGP*DC)-3' in PDB entry 3ebc, assembly 1, side view.
5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DGP*DGP*DGP*DC)-3' in PDB entry 3ebc, assembly 1, top view.
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DNA chain F
Name: 5'-D(*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3'
Representative chains: F
Source organism: Haemophilus influenzae [727]
Expression system: Not provided
Length: 14 nucleotides
Theoretical weight: 4.24 KDa
5'-D(*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3' in PDB entry 3ebc, assembly 1, front view.
5'-D(*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3' in PDB entry 3ebc, assembly 1, side view.
5'-D(*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3' in PDB entry 3ebc, assembly 1, top view.
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