PDBe 3ebc

X-ray diffraction
2.55Å resolution

Structure of N141A HincII with Cognate DNA

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Type-2 restriction enzyme HincII Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 36.59 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 1-258; Coverage: 100%)
Gene name: hincIIR
Sequence domains: Restriction endonuclease HincII
Structure domains: ECO RV Endonuclease; Chain A
5'-D(*DGP*DCP*DCP*DCP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3' Chain: F
Molecule details ›
Chain: F
Length: 14 nucleotides
Theoretical weight: 4.24 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DGP*DGP*DGP*DC)-3' Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.32 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 46.551Å b: 91.571Å c: 70.594Å
α: 90° β: 107.46° γ: 90°
R-values:
R R work R free
0.28 0.191 0.279
Expression systems:
  • Escherichia coli
  • Not provided