› 3ea3

X-ray diffraction
1.78Å resolution

Crystal Structure of the Y246S/Y247S/Y248S/Y251S Mutant of Phosphatidylinositol-Specific Phospholipase C from Bacillus Thuringiensis

Released:
DOI: 10.2210/pdb3ea3/pdb
PDB entry 3ea3 coloured by chain, front view.
PDB entry 3ea3 coloured by chain, side view.
PDB entry 3ea3 coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Modulation of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C activity by mutations in the putative dimerization interface.
Shi X, Shao C, Zhang X, Zambonelli C, Redfield AG, Head JF, Seaton BA, Roberts MF
J Biol Chem 284 15607-18 (2009)
PMID: 19369255

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 34.25 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08954 (Residues: 32-329; Coverage: 100%)
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

1 bound ligand:
Ligand MN 5 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P1
Unit cell:
a: 47.666Å b: 56.352Å c: 59.852Å
α: 92.36° β: 99.38° γ: 113.21°
R-values:
R R work R free
0.185 0.185 0.221
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Cation-π interactions as lipid-specific anchors for phosphatidylinositol-specific phospholipase C.
Grauffel et al. (2013)
12 more

1 mention without citation

Modulation of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C activity by mutations in the putative dimerization interface.
Shi et al. (2009)