› 3ea1

X-ray diffraction
1.75Å resolution

Crystal Structure of the Y247S/Y251S Mutant of Phosphatidylinositol-Specific Phospholipase C from Bacillus Thuringiensis

Released:
DOI: 10.2210/pdb3ea1/pdb
PDB entry 3ea1 coloured by chain, front view.
PDB entry 3ea1 coloured by chain, side view.
PDB entry 3ea1 coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Modulation of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C activity by mutations in the putative dimerization interface.
Shi X, Shao C, Zhang X, Zambonelli C, Redfield AG, Head JF, Seaton BA, Roberts MF
J Biol Chem 284 15607-18 (2009)
PMID: 19369255

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 34.4 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08954 (Residues: 32-329; Coverage: 100%)
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 59.643Å b: 97.064Å c: 112.799Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.23
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

High-resolution NMR field-cycling device for full-range relaxation and structural studies of biopolymers on a shared commercial instrument.
Redfield AG. (2012)
8 more

6 mentions without citation

Cation-π interactions as lipid-specific anchors for phosphatidylinositol-specific phospholipase C.
Grauffel et al. (2013)
5 more