PDBe 3e8c

X-ray diffraction
2.2Å resolution

Crystal structures of the kinase domain of PKA in complex with ATP-competitive inhibitors

Released:
Source organism: Bos taurus

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase inhibitor alpha Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q3SX13 (Residues: 6-25; Coverage: 26%)
Gene name: PKIA
cAMP-dependent protein kinase catalytic subunit alpha Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 350 amino acids
Theoretical weight: 40.79 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00517 (Residues: 2-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 82.46Å b: 96.392Å c: 180.036Å
α: 90° β: 102.66° γ: 90°
R-values:
R R work R free
0.265 0.264 0.29
Expression systems:
  • Not provided
  • Escherichia coli