PDBe 3e7h

X-ray diffraction
1.7Å resolution

The crystal structure of the beta subunit of the DNA-directed RNA polymerase from Vibrio cholerae O1 biovar eltor

Released:
Source organism: Vibrio cholerae
Entry authors: Zhang R, Wu R, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.46 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KV30 (Residues: 228-329; Coverage: 8%)
Gene names: VC_0328, rpoB

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 65.035Å b: 65.035Å c: 115.981Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.229
Expression system: Escherichia coli BL21