› 3e5j

X-ray diffraction
1.95Å resolution

Crystal structure of CYP105P1 wild-type ligand-free form

Released:
DOI: 10.2210/pdb3e5j/pdb
PDB entry 3e5j coloured by chain, front view.
PDB entry 3e5j coloured by chain, side view.
PDB entry 3e5j coloured by chain, top view.
Source organism: Streptomyces avermitilis
Primary publication:
Crystal structures of cytochrome P450 105P1 from Streptomyces avermitilis: conformational flexibility and histidine ligation state.
Xu LH, Fushinobu S, Ikeda H, Wakagi T, Shoun H
J Bacteriol 191 1211-9 (2009)
PMID: 19074393

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-188055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.41 KDa
Source organism: Streptomyces avermitilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93H81 (Residues: 1-399; Coverage: 100%)
Gene names: SAV14893_091320, SAV31267_094820, pteC
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P43212
Unit cell:
a: 88.401Å b: 88.401Å c: 193.885Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.211
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Diversity of P450 enzymes in the biosynthesis of natural products.
Podust et al. (2012)
2 more

8 mentions without citation

Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function.
Rudolf et al. (2017)
7 more