PDBe 3e44

X-ray diffraction
2.52Å resolution

Q138F HincII bound to cleaved DNA (GTT | AAC) and Mn2+

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Type-2 restriction enzyme HincII Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.84 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 2-258; Coverage: 100%)
Gene name: hincIIR
Sequence domains: Restriction endonuclease HincII
Structure domains: ECO RV Endonuclease; Chain A
5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DT)-3' Chains: E, G
Molecule details ›
Chains: E, G
Length: 7 nucleotides
Theoretical weight: 2.13 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(P*DAP*DAP*DCP*DCP*DGP*DGP*DC)-3' Chains: F, H
Molecule details ›
Chains: F, H
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 50.694Å b: 91.788Å c: 66.18Å
α: 90° β: 105.3° γ: 90°
R-values:
R R work R free
0.184 0.18 0.257
Expression systems:
  • Escherichia coli
  • Not provided