PDBe 3e3f

X-ray diffraction
2.3Å resolution

H. influenzae beta-carbonic anhydrase, variant V47A with 100 mM bicarbonate

Released:

Function and Biology Details

Reaction catalysed:
H(2)CO(3) = CO(2) + H(2)O. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.26 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45148 (Residues: 1-229; Coverage: 100%)
Gene names: HI_1301, can
Sequence domains: Carbonic anhydrase
Structure domains: Beta-carbonic Anhydrase; Chain A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P41212
Unit cell:
a: 83.897Å b: 83.897Å c: 184.867Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.23
Expression system: Escherichia coli