PDBe 3e2e

X-ray diffraction
3Å resolution

Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 889 amino acids
Theoretical weight: 99.83 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
DNA (31-MER) Chain: T
Molecule details ›
Chain: T
Length: 33 nucleotides
Theoretical weight: 10.12 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (28-MER) Chain: N
Molecule details ›
Chain: N
Length: 33 nucleotides
Theoretical weight: 10.08 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*GP*GP*AP*GP*UP*G)-3') Chain: R
Molecule details ›
Chain: R
Length: 7 nucleotides
Theoretical weight: 2.32 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P61
Unit cell:
a: 81.008Å b: 81.008Å c: 358.791Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.24 0.292
Expression systems:
  • Escherichia coli
  • Not provided