PDBe 3e1v

X-ray diffraction
2.8Å resolution

H. influenzae beta-carbonic anhydrase, variant D44N

Released:

Function and Biology Details

Reaction catalysed:
H(2)CO(3) = CO(2) + H(2)O. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.29 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45148 (Residues: 1-229; Coverage: 100%)
Gene names: HI_1301, can
Sequence domains: Carbonic anhydrase
Structure domains: Beta-carbonic Anhydrase; Chain A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: P41212
Unit cell:
a: 81.574Å b: 81.574Å c: 187.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.233
Expression system: Escherichia coli