› 3e1i

X-ray diffraction
2.3Å resolution

Crystal Structure of BbetaD432A Variant Fibrinogen Fragment D with the Peptide Ligand Gly-His-Arg-Pro-amide

Released:
DOI: 10.2210/pdb3e1i/pdb
PDB entry 3e1i coloured by chain, front view.
PDB entry 3e1i coloured by chain, side view.
PDB entry 3e1i coloured by chain, top view.
Source organisms:
Primary publication:
Fibrinogen variant BbetaD432A has normal polymerization but does not bind knob "B".
Bowley SR, Lord ST
Blood 113 4425-30 (2009)
PMID: 19075185

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-136014 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Fibrinogen alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 87 amino acids
Theoretical weight: 10.24 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P02671 (Residues: 130-216; Coverage: 10%)
Gene name: FGA
Sequence domains: Fibrinogen alpha/beta chain family
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Fibrinogen beta chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 328 amino acids
Theoretical weight: 37.65 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P02675 (Residues: 164-491; Coverage: 71%)
Gene name: FGB
Sequence domains:
Structure domains:
Fibrinogen gamma chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 319 amino acids
Theoretical weight: 36.22 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P02679 (Residues: 114-432; Coverage: 75%)
Gene names: FGG, PRO2061
Sequence domains:
Structure domains:
Gly-His-Arg-Pro-amide Chains: G, H
Molecule details ›
Chains: G, H
Length: 5 amino acids
Theoretical weight: 466 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand CA 7 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 54.476Å b: 146.596Å c: 228.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.242
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

3 review citations

Mechanisms of fibrin polymerization and clinical implications.
Weisel et al. (2013)
2 more

9 mentions without citation

Streptococcal M1 protein constructs a pathological host fibrinogen network.
Macheboeuf et al. (2011)
8 more