PDBe 3e00

X-ray diffraction
3.1Å resolution

Intact PPAR gamma - RXR alpha Nuclear Receptor Complex on DNA bound with GW9662, 9-cis Retinoic Acid and NCOA2 Peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Nuclear receptor coactivator 2 Chains: E, G
Molecule details ›
Chains: E, G
Length: 13 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 685-697; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2
Retinoic acid receptor RXR-alpha Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 51.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19793 (Residues: 11-462; Coverage: 98%)
  • Best match: P19793-2 (Residues: 1-365)
Gene names: NR2B1, RXRA
Sequence domains:
Structure domains:
Peroxisome proliferator-activated receptor gamma Chain: D
Molecule details ›
Chain: D
Length: 419 amino acids
Theoretical weight: 48.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37231 (Residues: 102-505; Coverage: 80%)
Gene names: NR1C3, PPARG
Sequence domains:
Structure domains:
DNA (5'-D(*DCP*DAP*DAP*DAP*DCP*DTP*DAP*DGP*DGP*DTP*DCP*DAP*DAP*DAP*DGP*DGP*DTP*DCP*DAP*DG)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.18 KDa
DNA (5'-D(*DCP*DTP*DGP*DAP*DCP*DCP*DTP*DTP*DTP*DGP*DAP*DCP*DCP*DTP*DAP*DGP*DTP*DTP*DTP*DG)-3') Chain: F
Molecule details ›
Chain: F
Length: 20 nucleotides
Theoretical weight: 6.09 KDa

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 63.585Å b: 146.775Å c: 67.23Å
α: 90° β: 115.52° γ: 90°
R-values:
R R work R free
0.214 0.214 0.276
Expression systems:
  • Not provided
  • Escherichia coli