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E(L212)A, D(L213)A, N(M5)D triple mutant structure of photosynthetic reaction center from Rhodobacter sphaeroides

X-ray diffraction
2.7Å resolution
PDB entry 3duq coloured by chain, front view.
PDB entry 3duq coloured by chain, side view.
PDB entry 3duq coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Reaction Centre
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Multimeric state: hetero trimer
Accessible surface area: 29199.74 Å2
Buried surface area: 37014.49 Å2
Dissociation area: 905.45 Å2
Dissociation energy (ΔGdiss): 9.49 kcal/mol
Dissociation entropy (TΔSdiss): 7.75 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-142934

Macromolecules

Reaction center protein L chain
Chain: L
Length: 281 amino acids
Theoretical weight: 31.24 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Pfam: Photosynthetic reaction centre protein
InterPro:
CATH: Photosystem II protein D1-like
Reaction center protein L chain in PDB entry 3duq, assembly 1, front view.
Reaction center protein L chain in PDB entry 3duq, assembly 1, side view.
Reaction center protein L chain in PDB entry 3duq, assembly 1, top view.
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Reaction center protein M chain
Chain: M
Length: 314 amino acids
Theoretical weight: 35.37 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Pfam: Photosynthetic reaction centre protein
InterPro:
CATH: Photosystem II protein D1-like
Reaction center protein M chain in PDB entry 3duq, assembly 1, front view.
Reaction center protein M chain in PDB entry 3duq, assembly 1, side view.
Reaction center protein M chain in PDB entry 3duq, assembly 1, top view.
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Reaction center protein H chain
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Pfam:
InterPro:
CATH:
Reaction center protein H chain in PDB entry 3duq, assembly 1, front view.
Reaction center protein H chain in PDB entry 3duq, assembly 1, side view.
Reaction center protein H chain in PDB entry 3duq, assembly 1, top view.
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