PDBe 3du3

X-ray diffraction
2.8Å resolution

E(L212)A, D(L213)A, A(M249)Y triple mutant structure of photosynthetic reaction center

Released:
Source organism: Rhodobacter sphaeroides
Entry authors: Pokkuluri PR, Schiffer M

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.24 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Membrane spanning alpha-helix pairs
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 314 amino acids
Theoretical weight: 35.46 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Membrane spanning alpha-helix pairs

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 141.1Å b: 141.1Å c: 187.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 0.201
Expression system: Rhodobacter sphaeroides