PDBe 3dqw

X-ray diffraction
2.02Å resolution

c-Src kinase domain Thr338Ile mutant in complex with ATPgS

Released:
Source organism: Gallus gallus
Primary publication:
Activation of tyrosine kinases by mutation of the gatekeeper threonine.
OpenAccess logo Nat. Struct. Mol. Biol. 15 1109-18 (2008)
PMID: 18794843

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 286 amino acids
Theoretical weight: 32.82 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 251-533; Coverage: 53%)
Gene name: SRC
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 82.42Å b: 103.65Å c: 83.69Å
α: 90° β: 103.94° γ: 90°
R-values:
R R work R free
0.207 0.205 0.246
Expression system: Escherichia coli