› 3dkh

X-ray diffraction
2.4Å resolution

L559A mutant of Melanocarpus albomyces laccase

Released:
DOI: 10.2210/pdb3dkh/pdb
PDB entry 3dkh coloured by chain, front view.
PDB entry 3dkh coloured by chain, side view.
PDB entry 3dkh coloured by chain, top view.
Source organism: Melanocarpus albomyces
Primary publication:
Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure.
Andberg M, Hakulinen N, Auer S, Saloheimo M, Koivula A, Rouvinen J, Kruus K
FEBS J 276 6285-300 (2009)
PMID: 19780817

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Laccase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 61.8 KDa
Source organism: Melanocarpus albomyces
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q70KY3 (Residues: 51-609; Coverage: 93%)
Gene name: LAC1
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
6 bound ligands:
Ligand NAG 6 x NAG
Ligand CU 8 x CU
Ligand CL 1 x CL
Ligand SO4 3 x SO4
Ligand OXY 2 x OXY
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: C2
Unit cell:
a: 173.51Å b: 62.02Å c: 125.67Å
α: 90° β: 99.92° γ: 90°
R-values:
R R work R free
0.224 0.224 0.282
Expression system: Saccharomyces cerevisiae

Quick links

Citations

10 review citations

Designer laccases: a vogue for high-potential fungal enzymes?
Rodgers et al. (2010)
9 more