PDBe 3dhw

X-ray diffraction
3.7Å resolution

Crystal structure of methionine importer MetNI

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methionine import ATP-binding protein MetN Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 343 amino acids
Theoretical weight: 37.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P30750 (Residues: 1-343; Coverage: 100%)
Gene names: JW0195, abc, b0199, metN
Sequence domains:
Structure domains:
D-methionine transport system permease protein MetI Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 217 amino acids
Theoretical weight: 23.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31547 (Residues: 1-217; Coverage: 100%)
Gene names: JW0194, b0198, metI, yaeE
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1, SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 97.7Å b: 165.4Å c: 289.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.31 0.31 0.347
Expression system: Escherichia coli