PDBe 3dfr

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURES OF ESCHERICHIA COLI AND LACTOBACILLUS CASEI DIHYDROFOLATE REDUCTASE REFINED AT 1.7 ANGSTROMS RESOLUTION. I. GENERAL FEATURES AND BINDING OF METHOTREXATE

Released:

Function and Biology Details

Reaction catalysed:
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.35 KDa
Source organism: Lactobacillus casei
Expression system: Not provided
UniProt:
  • Canonical: P00381 (Residues: 2-163; Coverage: 99%)
Gene names: dhfR, folA
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P61
Unit cell:
a: 71.86Å b: 71.86Å c: 93.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.152 not available
Expression system: Not provided