PDBe 3dep

X-ray diffraction
2.7Å resolution

Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
YPGGSFDPLGLA Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.19 KDa
Source organism: Synthetic construct
Expression system: Not provided
Signal recognition particle 43 kDa protein, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.04 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O22265 (Residues: 85-267; Coverage: 49%)
Gene names: At2g47450, CAO, CPSRP43, T30B22.25
Sequence domains: Ankyrin repeat
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P6122
Unit cell:
a: 66.473Å b: 66.473Å c: 219.703Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.249 0.298
Expression systems:
  • Not provided
  • Escherichia coli