PDBe 3dc0

X-ray diffraction
2.78Å resolution

Crystal structure of native alpha-amylase from Bacillus sp. KR-8104

Released:
Source organism: Bacillus sp. KR-8104
Entry authors: Alikhajeh J, Khajeh K, Ranjbar B, Naderi-Manesh M, Naderi-Manesh H, Chen CJ

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 46.64 KDa
Source organism: Bacillus sp. KR-8104
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B3GQD0 (Residues: 45-466; Coverage: 67%)
Gene name: kra2
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2221
Unit cell:
a: 140.176Å b: 163.141Å c: 73.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.233
Expression system: Escherichia coli BL21