› 3dc0

X-ray diffraction
2.78Å resolution

Crystal structure of native alpha-amylase from Bacillus sp. KR-8104

Released:
DOI: 10.2210/pdb3dc0/pdb
PDB entry 3dc0 coloured by chain, front view.
PDB entry 3dc0 coloured by chain, side view.
PDB entry 3dc0 coloured by chain, top view.
Source organism: Bacillus sp. KR-8104
Entry authors: Alikhajeh J, Khajeh K, Ranjbar B, Naderi-Manesh M, Naderi-Manesh H, Chen CJ

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 422 amino acids
Theoretical weight: 46.64 KDa
Source organism: Bacillus sp. KR-8104
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B3GQD0 (Residues: 45-466; Coverage: 64%)
Gene name: kra2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2221
Unit cell:
a: 140.176Å b: 163.141Å c: 73.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.233
Expression system: Escherichia coli BL21

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