PDBe 3dag

X-ray diffraction
1.75Å resolution

The crystal structure of [Fe]-hydrogenase holoenzyme (HMD) from METHANOCALDOCOCCUS JANNASCHII

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5,10-methenyltetrahydromethanopterin hydrogenase Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 38.71 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q58194 (Residues: 1-358; Coverage: 100%)
Gene names: MJ0784, hmd
Sequence domains: H2-forming N5,N10-methylene-tetrahydromethanopterin dehydrogenase
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I4122
Unit cell:
a: 95.93Å b: 95.93Å c: 165.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.22
Expression system: Escherichia coli