› 3d4f

X-ray diffraction
1.55Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase SHV-1 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 31.26 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AD64 (Residues: 1-286; Coverage: 100%)
Gene names: bla, blaSHV-1, shv1
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand MA4 2 x MA4
Ligand LN1 1 x LN1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 49.611Å b: 55.503Å c: 83.502Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.197
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Current challenges in antimicrobial chemotherapy: focus on ß-lactamase inhibition.
Bebrone et al. (2010)
5 more

13 mentions without citation

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
12 more