› 3cz4

X-ray diffraction
1.7Å resolution

Native AphA class B acid phosphatase/phosphotransferase from E. coli

Released:
DOI: 10.2210/pdb3cz4/pdb
PDB entry 3cz4 coloured by chain, front view.
PDB entry 3cz4 coloured by chain, side view.
PDB entry 3cz4 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural insights into the catalytic mechanism of the bacterial class B phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases.
Leone R, Cappelletti E, Benvenuti M, Lentini G, Thaller MC, Mangani S
J Mol Biol 384 478-88 (2008)
PMID: 18845157

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Class B acid phosphatase Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AE22 (Residues: 26-237; Coverage: 100%)
Gene names: JW4015, aphA, b4055, napA, yjbP
Sequence domains: HAD superfamily, subfamily IIIB (Acid phosphatase)
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand ACT 1 x ACT
Ligand CL 1 x CL
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: I222
Unit cell:
a: 49.294Å b: 92.775Å c: 137.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Recognition of nucleoside monophosphate substrates by Haemophilus influenzae class C acid phosphatase.
Singh et al. (2010)
2 more