PDBe 3cyf

X-ray diffraction
1.6Å resolution

Crystal Structure of E18N DJ-1

Released:

Function and Biology Details

Reaction catalysed:
(1) An N(omega)-(1-hydroxy-2-oxopropyl)-[protein]-L-arginine + H(2)O = a [protein]-L-arginine + (R)-lactate. (2) An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + (R)-lactate. (3) An S-(1-hydroxy-2-oxopropyl)-[protein]-L-cysteine + H(2)O = a [protein]-L-cysteine + (R)-lactate. 

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein DJ-1 Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 21.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3121
Unit cell:
a: 75.025Å b: 75.025Å c: 75.054Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.158 0.184
Expression system: Escherichia coli