› 3cxb

X-ray diffraction
2.6Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176317 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Secreted effector protein SifA Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 38.55 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56061 (Residues: 1-336; Coverage: 100%)
Gene names: STM1224, sifA
Sequence domains: Sif protein
Structure domains:
Pleckstrin homology domain-containing family M member 2 Chain: B
Molecule details ›
Chain: B
Length: 112 amino acids
Theoretical weight: 12.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IWE5 (Residues: 773-884; Coverage: 11%)
Gene names: KIAA0842, PLEKHM2, SKIP
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300, BSRF BEAMLINE 3W1A
Spacegroup: P21212
Unit cell:
a: 91.69Å b: 110.679Å c: 45.533Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.258 0.288
Expression system: Escherichia coli

Quick links

Citations

50 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
49 more

10 mentions without citation

Bacterial guanine nucleotide exchange factors SopE-like and WxxxE effectors.
Bulgin et al. (2010)
9 more