› 3cp4

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE CYTOCHROME P450-CAM ACTIVE SITE MUTANT THR252ALA

Released:
DOI: 10.2210/pdb3cp4/pdb
PDB entry 3cp4 coloured by chain, front view.
PDB entry 3cp4 coloured by chain, side view.
PDB entry 3cp4 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of the cytochrome P-450CAM active site mutant Thr252Ala.
Raag R, Martinis SA, Sligar SG, Poulos TL
Biochemistry 30 11420-9 (1991)
PMID: 1742281

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.59 KDa
Source organism: Pseudomonas putida
Expression system: Not provided
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand ADM 1 x ADM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 108.67Å b: 103.9Å c: 36.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 not available not available
Expression system: Not provided

Quick links

Citations

22 review citations

The molecular biology, biochemistry, and physiology of human steroidogenesis and its disorders.
Miller et al. (2011)
21 more

1 mention without citation

Identification of a functional water channel in cytochrome P450 enzymes.
Oprea et al. (1997)