› 3co5

X-ray diffraction
2.4Å resolution

Crystal structure of sigma-54 interaction domain of putative transcriptional response regulator from Neisseria gonorrhoeae

Released:
DOI: 10.2210/pdb3co5/pdb
PDB entry 3co5 coloured by chain, front view.
PDB entry 3co5 coloured by chain, side view.
PDB entry 3co5 coloured by chain, top view.
Source organism: Neisseria gonorrhoeae FA 1090
Entry authors: Osipiuk J, Hendricks R, Bigelow L, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen assimilation regulatory protein NtrX Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 15.65 KDa
Source organism: Neisseria gonorrhoeae FA 1090
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5F5Q1 (Residues: 133-272; Coverage: 33%)
Gene name: NGO_1866
Sequence domains: Sigma-54 interaction domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand BME 2 x BME
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P32
Unit cell:
a: 67.963Å b: 67.963Å c: 64.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.241
Expression system: Escherichia coli BL21(DE3)

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