PDBe 3cmu

X-ray diffraction
4.2Å resolution

Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures

Released:
Source organism: Escherichia coli K-12

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protein RecA Chain: A
Molecule details ›
Chain: A
Length: 2050 amino acids
Theoretical weight: 218.97 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P0A7G6 (Residues: 31-335, 2-335; Coverage: 95%)
Gene names: JW2669, b2699, lexB, recA, recH, rnmB, tif, umuB, zab
Sequence domains: recA bacterial DNA recombination protein
DNA (5'-D(*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DTP*DT)-3') Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.43 KDa

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 156Å b: 156Å c: 211Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.276 0.275 0.298
Expression system: Not provided