PDBe 3cmt

X-ray diffraction
3.15Å resolution

Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures

Released:
Source organism: Escherichia coli K-12

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein RecA Chains: A, D
Molecule details ›
Chains: A, D
Length: 1706 amino acids
Theoretical weight: 182.24 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P0A7G6 (Residues: 31-335, 2-335; Coverage: 95%)
Gene names: JW2669, b2699, lexB, recA, recH, rnmB, tif, umuB, zab
Sequence domains: recA bacterial DNA recombination protein
Structure domains: P-loop containing nucleotide triphosphate hydrolases
DNA (5'-D(P*DGP*DGP*DTP*DGP*DGP*DG)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 6 nucleotides
Theoretical weight: 1.91 KDa
DNA (5'-D(*DTP*DTP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DCP*DTP*DTP*DTP*DT)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 15 nucleotides
Theoretical weight: 4.45 KDa

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 159Å b: 300.5Å c: 80.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.217 0.243
Expression system: Not provided