› 3cgl

X-ray diffraction
2.09Å resolution

Crystal Structure and Raman Studies of dsFP483, a Cyan Fluorescent Protein from Discosoma striata

Released:
DOI: 10.2210/pdb3cgl/pdb
PDB entry 3cgl coloured by chain, front view.
PDB entry 3cgl coloured by chain, side view.
PDB entry 3cgl coloured by chain, top view.
Source organism: Discosoma striata
Primary publication:
Crystal structure and Raman studies of dsFP483, a cyan fluorescent protein from Discosoma striata.
Malo GD, Wang M, Wu D, Stelling AL, Tonge PJ, Wachter RM
J Mol Biol 378 871-86 (2008)
PMID: 18395223

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein dsFP483 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 241 amino acids
Theoretical weight: 27.77 KDa
Source organism: Discosoma striata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y7 (Residues: 1-232; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
1 modified residue:
Ligand CRQ 6 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: C2
Unit cell:
a: 111.202Å b: 78.269Å c: 188.566Å
α: 90° β: 91.35° γ: 90°
R-values:
R R work R free
0.186 0.185 0.217
Expression system: Escherichia coli

Quick links

Citations

1 review citation

GFP family: structural insights into spectral tuning.
Pakhomov et al. (2008)
2 other articles

3 mentions without citation

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.
Shaner et al. (2013)
2 more