› 3cfh

X-ray diffraction
1.75Å resolution

Photoswitchable red fluorescent protein psRFP, off-state

Released:
DOI: 10.2210/pdb3cfh/pdb
PDB entry 3cfh coloured by chain, front view.
PDB entry 3cfh coloured by chain, side view.
PDB entry 3cfh coloured by chain, top view.
Source organism: Anemonia sulcata
Entry authors: Kachalova GS, Gundel S, Bartunik HD, Wiedenmann J

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190440 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GFP-like non-fluorescent chromoprotein FP595 chain 1 Chains: L, M, R, S
Molecule details ›
Chains: L, M, R, S
Length: 62 amino acids
Theoretical weight: 6.74 KDa
Source organism: Anemonia sulcata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZ28 (Residues: 1-62; Coverage: 27%)
Structure domains: Pantoate--beta-alanine Ligase; Chain: A,domain 2
GFP-like non-fluorescent chromoprotein FP595 chain 2 Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 167 amino acids
Theoretical weight: 19.19 KDa
Source organism: Anemonia sulcata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZ28 (Residues: 63-232; Coverage: 73%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
2 modified residues:
Ligand NRQ 4 x NRQ
Ligand CME 8 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2221
Unit cell:
a: 97.819Å b: 97.851Å c: 241.827Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.23
Expression system: Escherichia coli

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