PDBe 3cbb

X-ray diffraction
2Å resolution

Crystal Structure of Hepatocyte Nuclear Factor 4alpha in complex with DNA: Diabetes Gene Product

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Hepatocyte nuclear factor 4-alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 78 amino acids
Theoretical weight: 9.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41235 (Residues: 58-135; Coverage: 17%)
Gene names: HNF4, HNF4A, NR2A1, TCF14
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
Hepatocyte Nuclear Factor 4-alpha promoter element DNA Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.41 KDa
Source organism: Synthetic construct
Expression system: Not provided
Hepatocyte Nuclear Factor 4-alpha promoter element DNA Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.48 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 121.628Å b: 35.425Å c: 70.985Å
α: 90° β: 119.36° γ: 90°
R-values:
R R work R free
0.213 0.213 0.252
Expression systems:
  • Escherichia coli
  • Not provided