PDBe 3bsh

X-ray diffraction
3Å resolution

Barley alpha-amylase isozyme 1 (AMY1) double mutant Y105A/Y380A in complex with inhibitor acarbose

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-amylase type A isozyme Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 45.31 KDa
Source organism: Hordeum vulgare
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00693 (Residues: 25-438; Coverage: 100%)
Gene name: AMY1.1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 99.5Å b: 72.1Å c: 60.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.268
Expression system: Komagataella pastoris