PDBe 3bg9

X-ray diffraction
3Å resolution

Crystal Structure of Human Pyruvate Carboxylase (missing the biotin carboxylase domain at the N-terminus) F1077A Mutant

Released:

Function and Biology Details

Reaction catalysed:
ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate carboxylase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 718 amino acids
Theoretical weight: 78.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11498 (Residues: 482-1178; Coverage: 59%)
Gene name: PC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 107.54Å b: 107.54Å c: 524.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.189 0.236
Expression system: Escherichia coli