PDBe 3beg

X-ray diffraction
2.9Å resolution

Crystal structure of SR protein kinase 1 complexed to its substrate ASF/SF2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SRSF protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 43.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96SB4 (Residues: 58-255, 474-655; Coverage: 58%)
Gene name: SRPK1
Sequence domains: Protein kinase domain
Structure domains:
Serine/arginine-rich splicing factor 1 Chain: B
Molecule details ›
Chain: B
Length: 115 amino acids
Theoretical weight: 13.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07955 (Residues: 105-219; Coverage: 46%)
Gene names: ASF, OK/SW-cl.3, SF2, SF2P33, SFRS1, SRSF1
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 57.406Å b: 117.525Å c: 193.554Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.298
Expression system: Escherichia coli BL21(DE3)