PDBe 3be1

X-ray diffraction
2.9Å resolution

Dual specific bH1 Fab in complex with the extracellular domain of HER2/ErbB-2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Fragment-Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab Fragment-Light Chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-2 Chain: A
Molecule details ›
Chain: A
Length: 624 amino acids
Theoretical weight: 68.79 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04626 (Residues: 23-646; Coverage: 51%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 62.324Å b: 115.056Å c: 208.171Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.236 0.277
Expression systems:
  • Escherichia coli
  • Cricetulus griseus