› 3b8m

X-ray diffraction
2.7Å resolution

Structure of FepE- Bacterial Polysaccharide Co-polymerase

Released:
DOI: 10.2210/pdb3b8m/pdb
PDB entry 3b8m coloured by chain, front view.
PDB entry 3b8m coloured by chain, side view.
PDB entry 3b8m coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Bacterial polysaccharide co-polymerases share a common framework for control of polymer length.
Tocilj A, Munger C, Proteau A, Morona R, Purins L, Ajamian E, Wagner J, Papadopoulos M, Van Den Bosch L, Rubinstein JL, Féthière J, Matte A, Cygler M
Nat Struct Mol Biol 15 130-8 (2008)
PMID: 18204465

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polysaccharide chain length determinant N-terminal domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 280 amino acids
Theoretical weight: 31.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XBV8 (Residues: 64-331; Coverage: 71%)
Gene names: ECs_0626, fepE
Sequence domains: Chain length determinant protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6322
Unit cell:
a: 139.726Å b: 139.726Å c: 276.654Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Pivotal roles of the outer membrane polysaccharide export and polysaccharide copolymerase protein families in export of extracellular polysaccharides in gram-negative bacteria.
Cuthbertson et al. (2009)
17 more

4 mentions without citation

ProSMoS server: a pattern-based search using interaction matrix representation of protein structures.
Shi et al. (2009)
3 more