› 3b1l

X-ray diffraction
1.85Å resolution

Crystal Structure of parkin ubiquitin-like domain R33Q mutant

Released:
DOI: 10.2210/pdb3b1l/pdb
PDB entry 3b1l coloured by chain, front view.
PDB entry 3b1l coloured by chain, side view.
PDB entry 3b1l coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Tomoo K, Ikemiya A, Amami Y, In Y, Ishida T

Function and Biology Details

Reaction catalysed: 
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase parkin Chain: X
Molecule details ›
Chain: X
Length: 76 amino acids
Theoretical weight: 8.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WVS6 (Residues: 1-76; Coverage: 16%)
Gene names: Park2, Prkn
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P61
Unit cell:
a: 45.79Å b: 45.79Å c: 65.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.188 0.243
Expression system: Escherichia coli

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