PDBe 3ayz

X-ray diffraction
1.22Å resolution

Membrane-bound respiratory [NiFe] hydrogenase from Hydrogenovibrio marinus in an air-oxidized condition


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hydrogenase Chains: A, C
Molecule details ›
Chains: A, C
Length: 596 amino acids
Theoretical weight: 66.57 KDa
Source organism: Hydrogenovibrio marinus
  • Canonical: F2Z6J6 (Residues: 1-596; Coverage: 98%)
Gene names: EI16_00020, hoxG
Sequence domains: Nickel-dependent hydrogenase
Structure domains: Cytochrome-c3 Hydrogenase, chain B
Membrane-bound hydrogenase small subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 283 amino acids
Theoretical weight: 31.31 KDa
Source organism: Hydrogenovibrio marinus
  • Canonical: F2Z6J5 (Residues: 41-323; Coverage: 78%)
Gene name: hoxK
Sequence domains:
Structure domains:

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 75.646Å b: 116.664Å c: 113.612Å
α: 90° β: 91.34° γ: 90°
R R work R free
0.139 not available 0.171