› 3ax6

X-ray diffraction
2.2Å resolution

Crystal structure of N5-carboxyaminoimidazole ribonucleotide synthetase from Thermotoga maritima

Released:
DOI: 10.2210/pdb3ax6/pdb
PDB entry 3ax6 coloured by chain, front view.
PDB entry 3ax6 coloured by chain, side view.
PDB entry 3ax6 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Entry authors: Miyazawa R, Kanagawa M, Baba S, Nakagawa N, Ebihara A, Kuramitsu S, Yokoyama S, Kawai G, Sampei G, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 380 amino acids
Theoretical weight: 43.13 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYS8 (Residues: 1-380; Coverage: 100%)
Gene names: TM_0447, purK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 4 x ADP
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21
Unit cell:
a: 63.919Å b: 186.59Å c: 68.463Å
α: 90° β: 90.49° γ: 90°
R-values:
R R work R free
0.216 0.216 0.268
Expression system: Escherichia coli

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