PDBe 3ax3

X-ray diffraction
2.1Å resolution

Crystal structure of rat TOM20-ALDH presequence complex: a complex (form2) between Tom20 and a disulfide-bridged presequence peptide containing D-Cys and L-Cys at the i and i+3 positions.

Released:

Function and Biology Details

Reaction catalysed:
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH. 
Biochemical function:
  • not assigned
Biological process:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aldehyde dehydrogenase, mitochondrial Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 12 amino acids
Theoretical weight: 1.25 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P11884 (Residues: 12-20; Coverage: 2%)
Gene name: Aldh2
Mitochondrial import receptor subunit TOM20 homolog Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 73 amino acids
Theoretical weight: 8.01 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62760 (Residues: 59-126; Coverage: 47%)
Gene name: Tomm20
Structure domains: Mitochondrial Import Receptor Subunit Tom20; Chain A

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: R32
Unit cell:
a: 99.596Å b: 99.596Å c: 195.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.243 0.287
Expression systems:
  • Not provided
  • Escherichia coli