› 3ar9

X-ray diffraction
2.6Å resolution

Calcium pump crystal structure with bound BeF3, TNP-AMP and TG in the absence of calcium

Released:
DOI: 10.2210/pdb3ar9/pdb
PDB entry 3ar9 coloured by chain, front view.
PDB entry 3ar9 coloured by chain, side view.
PDB entry 3ar9 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca2+-ATPase in the absence of Ca2+.
Toyoshima C, Yonekura S, Tsueda J, Iwasawa S
Proc Natl Acad Sci U S A 108 1833-8 (2011)
PMID: 21239683

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

5 bound ligands:
Ligand NA 1 x NA
Ligand MG 1 x MG
Ligand BEF 1 x BEF
Ligand TM1 1 x TM1
Ligand TG1 1 x TG1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 90.388Å b: 135.807Å c: 105.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.257

Quick links

Citations

6 review citations

Ion pathways in the sarcoplasmic reticulum Ca2+-ATPase.
Bublitz et al. (2013)
5 more

2 mentions without citation

Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca2+-ATPase in the absence of Ca2+.
Toyoshima et al. (2011)
1 more