› 3ap4

X-ray diffraction
2.33Å resolution

Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose

Released:
DOI: 10.2210/pdb3ap4/pdb
PDB entry 3ap4 coloured by chain, front view.
PDB entry 3ap4 coloured by chain, side view.
PDB entry 3ap4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Galectin-8-N-domain recognition mechanism for sialylated and sulfated glycans.
Ideo H, Matsuzaka T, Nonaka T, Seko A, Yamashita K
J Biol Chem 286 11346-55 (2011)
PMID: 21288902

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 154 amino acids
Theoretical weight: 17.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00214 (Residues: 1-154; Coverage: 49%)
Gene name: LGALS8
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P1
Unit cell:
a: 41.528Å b: 64.888Å c: 71.584Å
α: 98.41° β: 105.49° γ: 108.66°
R-values:
R R work R free
0.194 0.191 0.251
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Multifarious roles of sialic acids in immunity.
Varki et al. (2012)
22 more

5 mentions without citation

Galectin-8-N-domain recognition mechanism for sialylated and sulfated glycans.
Ideo et al. (2011)
4 more