PDBe 3aji

X-ray diffraction
2.05Å resolution

Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
26S proteasome non-ATPase regulatory subunit 10 Chains: A, C
Molecule details ›
Chains: A, C
Length: 231 amino acids
Theoretical weight: 25.18 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Z2X2 (Residues: 1-231; Coverage: 100%)
Gene name: Psmd10
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
26S proteasome regulatory subunit 6B Chains: B, D
Molecule details ›
Chains: B, D
Length: 83 amino acids
Theoretical weight: 9.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54775 (Residues: 337-418; Coverage: 20%)
Gene names: Psmc4, Tbp7
Structure domains: Helicase, Ruva Protein; domain 3

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU, SPRING-8 BEAMLINE BL26B2
Spacegroup: R3
Unit cell:
a: 103.164Å b: 103.164Å c: 154.993Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.171 0.229
Expression system: Escherichia coli