› 3afp

X-ray diffraction
2.05Å resolution

Crystal structure of the single-stranded DNA binding protein from Mycobacterium leprae (Form I)

Released:
DOI: 10.2210/pdb3afp/pdb
PDB entry 3afp coloured by chain, front view.
PDB entry 3afp coloured by chain, side view.
PDB entry 3afp coloured by chain, top view.
Source organism: Mycobacterium leprae TN
Primary publication:
X-ray and molecular-dynamics studies on Mycobacterium leprae single-stranded DNA-binding protein and comparison with other eubacterial SSB structures.
Kaushal PS, Singh P, Sharma A, Muniyappa K, Vijayan M
Acta Crystallogr D Biol Crystallogr 66 1048-58 (2010)
PMID: 20944238

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded DNA-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 168 amino acids
Theoretical weight: 17.72 KDa
Source organism: Mycobacterium leprae TN
Expression system: Escherichia coli
UniProt:
  • Canonical: P46390 (Residues: 1-168; Coverage: 100%)
Gene names: ML2684, MLCB1913.20c, ssb
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand CD 1 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 78.957Å b: 78.957Å c: 80.213Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.204 0.232
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Generation and Repair of Postreplication Gaps in Escherichia coli.
Cox et al. (2023)
7 other articles

2 mentions without citation

Structure-Guided Computational Approaches to Unravel Druggable Proteomic Landscape of Mycobacterium leprae.
Vedithi et al. (2021)
1 more