PDBe 3acc

X-ray diffraction
2.16Å resolution

Crystal structure of hypoxanthine-guanine phosphoribosyltransferase with GMP from Thermus thermophilus HB8

Released:
Source organism: Thermus thermophilus HB8
Primary publication:
Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) from Thermus thermophilus HB8.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 893-8 (2010)
PMID: 20693661

Function and Biology Details

Reaction catalysed:
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.19 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLS3 (Residues: 1-181; Coverage: 100%)
Gene name: TTHA0220
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P6522
Unit cell:
a: 67.139Å b: 67.139Å c: 152.543Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.21 0.25
Expression system: Escherichia coli